Laboratory for Sensory Circuit Formation
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Protocols & Resources


Optical Clearing using SeeDB (Ke et al., 2013)

SeeDB Resources

日本語の解説とプロトコル(簡易版)はこちら



Single-cell microarray (Imai et al., 2009)

overview

Single cell preparation

T7 promoter-liked cDNA synthesis from single cells

Cy Dye Labeling (for Agilent)

Hybridization (Agilent 4x44k)

*Quartz-Seq is an updated version of single cell transcriptome analysis based on our original protocol.



Single-cell cDNA library (Imai et al., 2006)

Single cell cDNA library (簡易法)



Two-color in situ hybridization (Imai et al., 2006; Imai et al., 2009)

Two-color in situ hybridization (TSA/TSA)

Two-color in situ hybridization (TSA/FastRed)

IHC/ISH共染色


その他

BAC manipulation

マウスジェノタイピング簡易法

抗原不活化(抗体染色用)


Tg mice (Imai et al., 2006; Imai et al., 2009)

I7-IRES-gapCFP

I7-IRES-Cre

I7(RDY)-IRES-gapYFP

caGs-IRES-gapYFP

I7(RDY)-IRES-caGs-IRES-gapCFP

I7-IRES-dnPKA-IRES-gapYFP

MOR23(del)-Venus

MOR23(del)-Cre-gapCFP line 17

MOR23(del)-Cre-gapCFP line 6 (class II-Cre)

I7(RDY)-IRES-Nrp1-IRES-gapCFP もしくは こちら

Gng8 KO

  

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